Ym1 protein crystals promote type 2 immunity

Ym1 protein crystals promote type 2 immunity

Blog Article

Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich Console Access Cover inflammation.In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eosinophils.Although mice do not encode Gal10 in their genome, they do form pseudo-CLCs, made from the chitinase-like proteins Ym1 and/or Ym2, encoded by Chil3 and Chil4 and made by myeloid and epithelial cells respectively.Here, we investigated the biological effects of pseudo-CLCs since their function is currently unknown.

We produced recombinant Ym1 crystals which were shown to have identical crystal packing and structure by X-ray crystallography as in vivo native crystals derived from murine lung.When administered to the airways of mice, crystalline but not soluble Ym1 stimulated innate and adaptive immunity and acted as a type 2 immune adjuvant for eosinophilic inflammation via triggering of dendritic cells (DCs).Murine Ym1 protein crystals found at sites of eosinophilic inflammation reinforce type 2 immunity and could serve as a surrogate model for studying Bagel the biology of human CLCs.